Orginal Research
2017 December
Volume : 5 Issue : 4


A comparative study of AFB smear and GeneXpert MTB/RIF assay in pulmonary and extrapulmonary specimens and detection of rifampicin resistance in a tertiary care hospital

Pragya Rani, Anil Kumar Bilolikar, Vishala C Sarma, Udayasri B, Sukrutha Gopal Reddy, Prasanna Lakshmi Kakarla, Ratna Shukla and Poonam AR

Pdf Page Numbers :- 115-120

Pragya Rani1,*, Anil Kumar Bilolikar1, Vishala C Sarma1, Udayasri B1, Sukrutha Gopal Reddy1, Prasanna Lakshmi Kakarla1, Ratna Shukla1 and Poonam AR1

 

1Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India

 

*Corresponding author: Dr. Pragya Rani, Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: drpragyarani@gmail.com

 

Received 10 August 2017; Revised 11 September 2017; Accepted 21 September 2017; Published 29 September 2017

 

Citation: Rani P, Bilolikar AK, Sarma VC, Udayasri B, Reddy SG, Kakarla PL, Shukla R, Poonam AR. A comparative study of AFB smear and GeneXpert MTB/RIF assay in pulmonary and extrapulmonary specimens and detection of rifampicin resistance in a tertiary care hospital. J Med Sci Res. 2017; 5(4):115-120. DOI: http://dx.doi.org/10.17727/JMSR.2017/5-22

 

Copyright: © 2017 Rani P, et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Tuberculosis is a major public health problem worldwide including India causing increased morbidity and mortality. There is a need for rapid identification for early diagnosis and treatment. In this study, GeneXpert MTB/RIF assay is used for rapid identification and was compared with AFB staining (by Zeihl Neelsen method). GeneXpert was used for Mycobacterium tuberculosis (MTB) complex identification and rifampicin detection from smear positive and smear negative patients. Out of 320 patients, 19 patients were acid-fast bacilli (AFB) smear positive and 67 patients detected for MTB by MTB/RIF assay. Rifampicin resistance (RR) was detected in 2 cases out of 62 positive cases by MTB/RIF assay. The test is considered more sensitive for AFB smear positive and showed high level of detection of MTB but less sensitive for AFB smear negative and showed low and medium level of detection of MTB. The GeneXpert is considered as an effective diagnostic tool for rapid diagnosis of MTB and RR in smear negative and positive specimens.

 

Keywords: Mycobacterium tuberculosis; GeneXpert MTB/RIF assay; rifampicin resistance; acid fast bacilli staining; pulmonary; extrapulmonary

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