Volume : 8
Issue : 1
A study of Candida albicans and non-albicans Candida species isolated from various clinical samples and their antifungal susceptibility pattern
Ratna Shukla, Sukrutha Gopal Reddy, Anil Kumar Bilolikar
Pdf Page Numbers :- 1-11
Ratna Shukla1,*, Sukrutha Gopal Reddy1 and Anil Kumar Bilolikar1
1Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India
*Corresponding author: Dr. Ratna Shukla, Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: firstname.lastname@example.org
Received 06 November 2019; Revised 14 December 2019; Accepted 23 December 2019; Published 30 December 2019
Citation: Shukla R, Reddy SG, Bilolikar AK. A study of Candida albicans and non-albicans Candida species isolated from various clinical samples and their antifungal susceptibility pattern. J Med Sci Res. 2020; 8(1):1-11. DOI: http://dx.doi.org/10.17727/JMSR.2020/8-1
Copyright: © 2020 Shukla R et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Background: Candida species are the most common cause of fungal infections worldwide. Candida albicans has been the most common causative agent until recent past but frequency of non-albicans Candida (NAC) species is on the rise. This changing epidemiology and increase in resistance to antifungal agents makes it important to identify Candida up to the species level and know its antifungal susceptibility pattern.
Aim: To provide data on Candida species prevalence and to highlight the need of speciation of Candida and its antifungal susceptibility testing.
Material and methods: For blood cultures, BacT/ALERT blood culture automated system (BioMérieux) was used. Candida was identified initially by direct microscopic examination of samples received and culture was done on SDA with antibiotic. Differentiation of Candida albicans and NAC species was done by germ tube test and final identification and antifungal susceptibility testing were done using Vitek 2 compact.
Result: The prevalence of Candida infection in this study is 1.58%. Out of total 100 Candida isolates, 25% were C.albicans and 75% were NAC. Among 75 non-albicans Candida spp.; C.tropicalis (46) forms the major isolate. Other NAC spp. included C.hemulonii (9), C.glabrata (6) & others (14). C.albicans is found to be more susceptible to fluconazole as compared to NAC spp. In this study NAC species showed comparatively reduced susceptibility to fluconazole and amphotericin B.
Conclusion: There is a significant epidemiological shift in candidiasis cases due to NAC species. Based on the present results and trends, it becomes essential to routinely identify Candida isolates up to species level, and detect evolving resistant strains by antifungal susceptibility testing wherever feasible.
Keywords: Non-albicans Candida; Candida auris; emerging infection; antifungal resistance