Volume : 6
Issue : 4
Diagnostic dilemma in extra-pulmonary tuberculosis: PCR a convenient tool
Ratna Shukla, Anil Kumar Bilolikar, Sukrutha Gopal Reddy
Pdf Page Numbers :- 97-102
Ratna Shukla1,*, Anil Kumar Bilolikar1 and Sukrutha Gopal Reddy1
1Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India
*Corresponding author: Dr. Ratna Shukla, Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: email@example.com
Received 17 July 2018; Revised 31 August 2018; Accepted 10 September 2018; Published 18 September 2018
Citation: Shukla R, Bilolikar AK, Reddy SG. Diagnostic dilemma in extra-pulmonary tuberculosis: PCR a convenient tool. J Med Sci Res. 2018; 6(4):97-102. DOI: http://dx.doi.org/10.17727/JMSR.2018/6-17
Copyright: © 2018 Shukla R et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
View Full Text
Background: Mycobacterium tuberculosis infection usually manifests as pulmonary tuberculosis and rarely as extra-pulmonary tuberculosis (EPTB). In recent past, there is an increase in the cases of extra-pulmonary tuberculosis. The diagnostic difficulty owing to paucibacillary nature of EPTB poses a challenge in diagnosis and consequently treatment. In this scenario, Polymerase chain reaction (PCR) serves as a convenient tool which gives results within a short time along with other conventional methods in the diagnosis of extra-pulmonary tuberculosis.
Aims: To detect Mycobacterium tuberculosis complex (MTBC) by DNA PCR in clinically suspected cases of EPTB.
Materials and methods: Clinical samples from suspected cases of EPTB were collected and processed according to the standard guidelines. Smears prepared were subjected to Ziehl-Neelsen (ZN) staining. Nested PCR was performed on extracted DNA from homogenized samples using commercial kit with MTB target gene of IS6110.
Results: A total of 156 samples were received for molecular diagnosis of EPTB over a year. They were all subjected to ZN staining and PCR test. All 156 samples were negative for AFB by ZN staining. Out of total 156 samples, 8(5.12%) samples were detected positive by MTB-DNA PCR test. The most common site of EPTB found in this study was pleura (14.28%). Other sites included genito-urinary tract (13.33%), CNS (4.65%) and endometrium (3.44%).
Conclusion: Early diagnosis and treatment of EPTB reduces complications and morbidity in affected patients. Though PCR is expensive; in diagnostic dilemma in suspected cases of EPTB, it can be included in diagnostic panel to clinch the diagnosis early and start pre-emptive treatment.
Keywords: Extra-pulmonary tuberculosis; MTB DNA-PCR; ZN staining; MTBC