Association of CTLA-4 A/G polymorphism with occurrence of ocular squamous cell carcinoma

Aparna Dwibedy, Sheetal Bakshi, Jayanti Mania Pramanik, Vidyashankar B, Avinash Funde Mahadeo, Priyanka Wagh

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 Aparna Dwibedy^1,*, Sheetal Bakshi², Jayanti Mania Pramanik³, Vidyashankar B⁴, Avinash Funde Mahadeo⁵ and Priyanka Wagh⁶

¹Department of Opthalmology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India

²Department of Ophthalmology, Sanjeevani eye hospital, Vitthalwadi, Ulhasnagar, Maharashtra 421003

³Department of Infectious Diseases Biology, National Institute for Research in Reproductive Health (ICMR), Parel, Mumbai-400012

⁴ Department of Ophthalmology, K. B. Haji Bacchooali Eye and ENT hospital, Parel, Mumbai-400012

⁵Department of Ophthalmology, Yashodeep superspeciality netralaya, Swami samarth road, Pathardi, Ahmednagar, Maharastra 414102

⁶Department of Infectious Diseases Biology, NIRRH, Parel East, Mumbai, Maharashtra 400012

*Corresponding author: Dr. Aparna Dwibedy, Consultant Orbit and Oculoplasty, Department of Opthalmology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: aparnadwibedy84@gmail.com

Received 09 September 2016; Revised 19 November 2016; Accepted 28 November 2016; Published 08 December 2016

Citation: Dwibedy A. Bakshi S, Pramanik JM, Vidyashankar B, Mahadeo AF, Wagh P. Association of CTLA-4 A/G polymorphism with occurrence of ocular squamous cell carcinoma. J Med Sci Res. 2017; 5(1):1-4. DOI: http://dx.doi.org/10.17727/JMSR.2017/5-1

Copyright: © 2017 Dwibedy A. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Introduction: The association of polymorphisms of genes controlling the immune regulation with development of cancers and autoimmune diseases, has been a heavily researched area with the hope of probable discovery of a biomarker or antitumour vaccine. The most commonly investigated polymorphism is CTLA-4 +49A/G. However, no research has been carried out to find out association between eye cancers and the same polymorphism till date, to the best of our knowledge.

Purpose: To find out association between CTLA-4 +49A/G polymorphism and ocular surface squamous neoplasia (OSSN).

Methods: 12 histologically proven OSSN patients were taken as subjects. 20 cataract patients were taken for comparison. This data was further compared with the published data on 103 individuals from an Indian study. DNA extraction, SNP selection and genotyping was done. Odds ratio, confidence interval and statistical significance were found out.

Results: 95% confidence interval was 0.044-1.521 between OSSN cases and cataract group (p=0.144). 95% confidence interval of the OSSN cases and healthy control group was 0.14-2.375 (p=0.544).

Conclusions: Statistically significant difference was not found in the frequency of polymorphism between OSSN cases and groups taken for comparison, in this pilot study. Larger number of OSSN cases need to be evaluated for the polymorphism.

Keywords: CTLA4; OSSN; +49 A/G Polymorphism; PCR-RLFP; Immune regulation