Original Research
2020 March
Volume : 8 Issue : 1


Comparative study of low density lipoprotein by estimation and calculation from lipid profile in a tertiary hospital

Lavanya Lagisetty, Radhika Chowdary Darapuneni

Pdf Page Numbers :- 12-16

Lavanya Lagisetty1, * and Radhika Chowdary Darapuneni1

 

1Department Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India

 

*Corresponding author: Dr. Lavanya Lagisetty, MD (Biochemistry), Department Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: lavanyamacha99@gmail.com

 

Received 11 November 2019; Revised 12 December 2019; Accepted 20 December 2019; Published 28 December 2019

 

Citation: Lagisetty L, Darapuneni RC. Comparative study of low density lipoprotein by estimation and calculation from lipid profile in a tertiary hospital. J Med Sci Res. 2020; 8(1):12-16. DOI: http://dx.doi.org/10.17727/JMSR.2020/8-2

 

Copyright: © 2020 Lagisetty L et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

View Full Text | PDF

Abstract

Introduction: Low-density lipoprotein cholesterol (LDL-C) is widely recognized as an established cardiovascular risk marker. Recent studies have shown that Friedewald underestimates LDL-C at lower levels, which could result in under treatment of high-risk patients of Atherosclerotic cardiovascular disease (ASCVD). A novel method Martin/Hopkins (LDL-CN) using a patient-specific conversion factor provides more accurate calculated LDL-C levels, LDL-C estimation is very important in high-risk patients to consider therapy.

Aims: To compare LDL-C values by using two formulae and direct estimation.

Materials and methods: A prospective study was undertaken in Department of Biochemistry in 1393 subjects from Dec 2018 to March 2019 with mean age of (53.23±13.36). LDL-C was calculated by using Friedewald formula LDL-C (LDL-CF) and Martin/Hopkins equation (LDL-CN) in 1000 subjects with Triglycerides (TG) <400mg/dl and for 393 patients with TG>400 ldl was directly estimated (LDL-CD) with kit in Beckman coulter Dxc860i.

Results: For this analysis, the mean age was (53.23±13.36). In 1000 patients, with TG<400mg/dl the mean LDL-CF (95.13±36.16mg/dl) was significantly lower compared to LDL-CN (98.90±35.88mg/dl) with p value (0.01). Thus, LDL-CN yielded a better diagnostic value than LDL-CF with regression of r2- 0.94. In 393 cases with Tg >400 mg/dl, the mean of LDL-CF (71.83±55.93mg/dl), was significantly lower compared to LDL-CD (104.17±41.6mg/dl) and LDL-CN (107.37±42.73mg/dl). p value was significant between Martin/Hopkins and Friedewald (<0.0001) and it is significant between LDL-CD and LDL-CF (<0.0001) but not between LDL-CD and LDL-CN (0.28).

Conclusion: This data suggest that Martin/Hopkins equation should be the preferred method to calculate LDL-C levels in with TG<400mg/dl and either direct measurement or Martin/Hopkins are the preferred methods in patients with TG>400mg/dl to treat high risk cases of ASCVD.

 

Keywords: Atherosclerotic cardiovascular disease; ASCVD; triglycerides; low density lipoprotein cholesterol

Subscription