Seroprevalance of HBsAg, anti-HIV 1&2 and anti-HCV by chemiluminescence method - A hospital based study from 2007 to 2014

Anil Kumar Bilolikar, Madhavi Latha B, Sukrutha Gopal Reddy, Vanaja S, Imtiyaz Ahmed MD, Radhika Chowdary D

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Anil Kumar Bilolikar^1,*, Madhavi Latha B¹, Sukrutha Gopal Reddy¹, Vanaja S¹, Imtiyaz Ahmed MD¹ and Radhika Chowdary D¹

¹Department of Laboratory Services. Krishna Institute of Medical Sciences, Secunderabad, Telangana.

*Corresponding author: Dr. Anil Kumar Bilolikar, Department of Laboratory Services, Krishna Institute of Medical Sciences, Secunderabad, Telangana. Email: dranilbilolikar@yahoo.com

Received 12 November 2014; Revised 10 December 2014; Accepted 17 December 2014; Published 27 December 2014

Citation: Anil Kumar B, Madhavi Latha B, Gopal Reddy S, Vanaja S, Imtiyaz Ahmed MD, Radhika Chowdary  D. Seroprevalance of HBsAg, anti-HIV 1&2 and anti-HCV by chemiluminescence method - A hospital based study from 2007 to 2014. J Med Sci Res 2015; 3(1):1-8. DOI: http://dx.doi.org/10.17727/JMSR.2015/3-001  

Copyright: © 2015 Anil Kumar B, et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Introduction: Hepatitis B virus (HBV), human immunodeficiency virus 1&2 (HIV1&2) and hepatitis C virus (HCV) are major public health problem. Detection of hepatitis B surface antigen (HBsAg), antibodies to human immunodeficiency virus 1&2 and hepatitis C virus in patients (both inpatients and outpatients) can help in patient management. Screening of patient samples for HBsAg, anti-HIV 1&2 and anti-HCV is important in managing surgical and medical care. The objective is to know prevalence of hepatitis B surface antigen (HBsAg), antibodies to HIV 1&2 and HCV inpatient and outpatient, their gender, age wise distribution and department wise distribution.

Material & methods: The present study was conducted in the Department of Microbiology, Krishna Institute of Medical Sciences (KIMS), Secunderabad, Telangana, during a study period of 8 years from January 2007 to December 2014. It is a cross sectional study. It is a laboratory based study, no clinical correlation has been done. The positive/reactive samples were studied for their gender, age group and department. Analysis was done on a yearly basis. A cumulative data of 8 years is being presented. The samples were screened by enhanced chemiluminescence (ECLIA) technology by using an instrument, Vitros ECiQ(Ortho Clinical Diagnostic).

Results: 1,00,590 patients were screened 1,805 (1.79 %) were positive for HBsAg. 92,819 patients screened 501(0.53%) were reactive for HIV 1&2 antibodies. 61,072 screened 744(1.21%) were reactive for HCV infection. The seroprevalence of all viral markers is more in males than females. In the present study seropositivity of HBsAg was higher in 41-50 year & 51-60 years followed by 31-40 years & 61-70 years. Highest prevalence of HIV was observed in 31-40 years followed by 41-50 years. Seropositivity of HCV was higher in 51-60 years followed by 41-50 years & 61-70 years.

Conclusion: Emphasizes the need of screening of viral markers for inpatients and out patients. Adherence to universal precautions

 Keywords: Seroprevalence of HBsAg; anti-HIV 1&2; anti-HCV; chemiluminescence method