Significance of hepatitis C virus (HCV) viremia in anti-HCV antibody (IgG) positive subjects

Sukrutha Gopal Reddy, Anil Kumar Bilolikar, Prasanna Lakshmi Kakarla

Pdf Page Numbers :- 17-20

 Sukrutha Gopal Reddy^1*, Anil Kumar Bilolikar², Prasanna Lakshmi Kakarla²

¹Department of Molecular Biology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India

²Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India

*Corresponding author: Dr. Sukrutha Gopal Reddy, Department of Molecular Biology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Mobile: 9177111719; Email: sukruthagopalreddy@gmail.com

Received 23 September 2016; Revised 18 November 2016; Accepted 30 November 2016; Published 19 December 2016

Citation: Reddy SG, Bilolikar AK, Kakarla PL. Significance of hepatitis C virus (HCV) viremia in anti-HCV antibody (IgG) positive subjects. J Med Sci Res. 2017; 5(1):17-20. DOI: http://dx.doi.org/10.17727/JMSR.2017/5-4

Copyright: © 2017 Reddy SG, et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Introduction: Hepatitis C is one of the most common bloodborne diseases causing significant morbidity and mortality globally.

Objective: This study was designed to evaluate the correlation between 3^rd generation ELISA positives for anti-HCV antibodies with real time PCR, among various categories of patients attending KIMS hospital.

Methods: This is a hospital based retrospective study at KIMS hospital, Secunderabad, India conducted between August 2013 and July 2015. From laboratory data, 115 patients, found to be anti-HCV antibody positive (done by CLIA method) were included in the study. Real-Time Reverse Transcription-PCR (RT-PCR) for hepatitis C virus (HCV) was done for these patient samples subsequently.

Results: Among a total of 115 anti-HCV antibody positive cases, 70(60.86%) were positive and 45(39.13%) were negative by HCV RNA PCR.

Conclusion: ELISA assays have many advantages in diagnostic settings including ease of automation, ease of use, relative cost effectiveness and low variability. Additional or confirmatory testing like RT-PCR is often helpful. The HCV RNA test is still regarded as a standard in the detection of active infection and for monitoring treatment.

Keywords: Anti-HCV antibody, RT-PCR, Horseradish peroxidase (HRP), Chemiluminiscent Immuno assay (CLIA).