Original Research
2015
December
Volume : 3
Issue : 4
A study of Acinetobacter from various clinical specimens & its antibiotic sensitivity pattern in a tertiary care hospital
Pragya Rani, Madhavi Latha B, Sukrutha Gopal Reddy, Anil Kumar Bilolikar
Pdf Page Numbers :- 162-165
Pragya Rani1,*, Madhavi Latha B1, Sukrutha Gopal Reddy1 and Anil Kumar Bilolikar1
1Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India
*Corresponding author: Dr. Pragya Rani, Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: drpragyarani@gmail.com
Received 25 June 2015; Revised 10 August 2015; Accepted 19 August 2015; Accepted 26 August 2015
Citation: Pragya Rani, Madhavi Latha B, Sukrutha Gopal R, Anil Kumar B. A study of Acinetobacter from various clinical specimens & its antibiotic sensitivity pattern in a tertiary care hospital. J Med Sci Res. 2015; 3(4):162-165. DOI: http://dx.doi.org/10.17727/JMSR.2015/3-031
Copyright: © 2015 Pragya Rani, et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract
Background: Acinetobacter baumannii has emerged as a significant hospital pathogen, quickly becoming resistant to commonly prescribed antimicrobials.
Objectives: To isolate various species of Acinetobacter, to compare inpatients (ICU’s & wards) and outpatients isolates and to know it’s frequency from various clinical specimens.
Material and methods: The retrospective study is conducted in the department of Microbiology, Krishna Institute of Medical Sciences, Secunderabad, from January 2013 to December 2014. The various clinical specimens from inpatients and outpatients were included. The samples were processed as per the standard guidelines. Identification & antibiotic sensitivity testing was done by using GN and AST 281 cards (Vitek 2 compact, BioMerieux) respectively. MIC values of antibiotics were obtained and reporting was done as per the CLSI guidelines. The data was captured from the laboratory computer and analysed.
Results: A total of 496 Acinetobacter species were isolated from 2459 samples (20.17%) from the entire hospital, in which Acinetobacter baumannii was 462(93.16%), Acinetobacter lwoffii was 16(3.22%), Acinetobacter junii was 13(2.62%), Acinetobacter haemolyticus was 5(1.00%). Maximum isolates observed from endotracheal tube secretions (39.51%) followed by blood specimens (15.12%), sputum (12.70%), pus swab (8.66%), clean catch (5.84%) and others (18.17%).
Conclusions: In this study, Acinetobacter isolates showed multidrug resistant pattern mostly in inpatients and hence there is a need for emphasizing the importance of hand washing and use of disinfectants in prevention of transmission of infection in health care setups.
Keywords: Acinetobacter; multi-drug resistance; Acinetobacter baumannii; Acinetobacter haemolyticus; VITEK