Antimicrobial activity of leaves extracts against bacteria isolated from wound infections

Goris BMT, Sabahalkheir KG, Ibrahim AA, Ibrahim AA, Ishaq MM, Ibrahim LO

Pdf Page Numbers :- 114-120

Babbiker Mohammed Taher Goris^1,*, Khansa Gasim Sabahalkheir^2,*, Aisha Abd-albagi Ibrahim², Aisha Abd-albagi Ibrahim², Mayada Mohammed Ishaq² and Loui Osman Ibrahim²

¹Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and technology, Sudan

²Department of Microbiology, Faculty of Medical Laboratory science, Khartoum University, Sudan

*Corresponding authors: Babbiker Mohammed Taher Gorish, Ph.D. in Medical Microbiology, Department of Microbiology and Immunology - Faculty of Medical Laboratory Science, Omdurman Islamic University, Sudan. Email: qorish456@gmail.com, Khansa Gasim Sabahalkheir, Department of Microbiology, Faculty of Medical Laboratory science, Khartoum University, Sudan.

Received 28 March 2019; Revised 30 May 2020; Accepted 13 June 2020; Published 22 June 2020

Citation: Goris BMT, Sabahalkheir KG, Ibrahim AA, Ibrahim AA, Ishaq MM, Ibrahim LO. Antimicrobial activity of leaves extracts against bacteria isolated from wound infections. J Med Sci Res. 2020; 8(3):114-120. DOI: http://dx.doi.org/10.17727/JMSR.2020/8-16

Copyright: © 2020 Goris BMT et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Background: Lawsonia inermis (L. inermis) is perennial plant commonly called henna. It is frequently cultivated in Sudan. Beside its uses cosmetics for staining hands and as hairs dyes‚ it was reported to be useful in jaundice, enlargement of spleen, calculus affliction and skin disease.

Method: This descriptive study was done during the period from December 2014 to April 2015 in order to determine the invitro antimicrobial activity of L. inermis (henna) leaves extract against standard and clinical isolates from wound swabs, including Staphylococcus aureus, Escherichia coli, Proteus species, Klebsiella pneumonia, and Pseudomonas aeruginosa. These organisms were collected from different hospitals in Khartoum State including: Soba University Hospital, Military Teaching Hospital, and Laboratory Management Center. The in vitro antimicrobial susceptibly testing was performed using cup plate diffusion method. The activity of L. inermis Linn leaves extract was controlled with four reference antibiotics including gentamicin, oxacillin, ciprofloxacin, and impinim.

Results: When aqueous extract of L. inermis Linn examined against standard bacteria and clinical isolates result showed that all standard bacteria were inhibited at 100%, 50%, and 25% concentration. All clinical isolates were successfully inhibited at 100%, 50%, 25%, and 12.5%. In contrary, the activity of methanolic extract of L. inermis Linn against standard bacteria showed that all standard bacteria were inhibited at 100%, 50% concentration, However, the clinical isolates showed an inhibition rate various depending on the concentration of methanolic extract of L. inermis Linn with S. aureus being most sensitive isolate.

Conclusion: We conclude that aqueous and methanolic extract of henna exhibited antimicrobial activity against all types of tested organisms both clinical and standard isolates. But the aqueous extract shows superior inhibition ability than the methanolic.

Keywords: Lawsonia inermis; antimicrobial sensitivity test; aqueous extract; methanolic extract; inhibition zone; wound infections; standard strain; clinical isolates