Determination and distribution of various antinuclear antibodies in systemic lupus erythematosus patients by using immunoblot testing

Udayasri B, Prakasham S, Salma M, Bhulakshmi

Pdf Page Numbers :- 68-73

Udayasri B^1,*, Swathi Prakasham¹, Mujahabeen salma¹ and Bhulakshmi¹

¹Department of laboratory Medicine, Block-C, Yashoda Hospitals, Somajiguda, Hyderabad, Telengana, India

*Corresponding author: Dr. Udayasri B, Consultant Microbiologist, Department of Laboratory Medicine, Yashoda hospitals, Somajiguda, India. Mobile: 9247576467; Email: udayasree09@gmail.com

Received 18 April 2018; Revised 07 June 2018; Accepted 15 June 2018; Published 22 June 2018

Citation: Udayasri B, Prakasham S, Salma M, Bhulakshmi. Determination and distribution of various antinuclear antibodies in systemic lupus erythematosus patients by using immunoblot testing. J Med Sci Res. 2018; 6(3):68-73. DOI: http://dx.doi.org/10.17727/JMSR.2018/6-12

Copyright: © 2018 Udayasri B et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Background: Systemic lupus erythematosus (SLE) is defined as a multisystemic inflammation resulting from production autoantibodies against nuclear and cytoplasmic antigen.

Objective: The present study was undertaken to determine the distribution of various auto antibodies in SLE patients by line immuno assay.

Materials and methods: A prospective study conducted at Yashodha Hospital, Somajuguda, Hyderabad. A total number of 120 clinically diagnosed SLE patients serum samples were collected and they were subjected to indirect immunofluorescence testing (IIFT) to screen autoantibodies.

Results: Out of 120 patients 97 were males and 23 were females, predominant age group affected was 41-60 years. Out of 120 patients 99 were positive by IIFT. Predominant pattern in IIFT is nucleus homogenous (54.54%), followed by nucleus speckled (29.9%). Out of 120 patients 69 were positive by ANA immunoblot, in that the predominant autoantibody was anti dsDNA antibody (40.57%), followed by RO-52 (31.88%), SSA (30.43%). Out of 120 Samples 68 were positive by both IIFT and immunoblot, 31 were only positive by IIFT, 20 were negative by both and one was only positive by immunoblot.

Conclusion: The study stresses on using IIFT as screening test for SLE. It should be followed by ANA immunoblot to determine specific antibody, which inturn helps in diagnosis, evaluation and follow-up of SLE patient.

Keywords: systemic lupus erythematosus; autoantibodies; antinuclear antibodies; indirect immuno fluorescence testing; immunoblot testing