Evaluation of clinicopathological parameters and immunoexpression of heat shock protein -27 in breast carcinoma and metastatic lymph nodes

Saroha N, Adil SAK

Pdf Page Numbers :- 211-217

Neha Saroha¹ and Adil SAK^1,*

¹Department of Pathology, Mysore Medical College and Research Institute, Mysuru, Karnataka 570001, India

*Corresponding author: Dr. SAK. Adil, Associate Professor, Department of Pathology, Mysore Medical College and Research Institute, Mysore, Karnataka 570001, India. Email: saka291@yahoo.co.in

Received 21 November 2024; Revised 6 March 2025; Accepted 15 March 2025; Published 21 March 2025

Citation: Saroha N, Adil SAK. Evaluation of clinicopathological parameters and immunoexpression of heat shock protein -27 in breast carcinoma and metastatic lymph nodes. J Med Sci Res. 2025; 13(2):211-217. DOI: http://dx.doi.org/10.17727/JMSR.2024/13-37

Copyright: © 2025 Saroha N et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Background: Breast cancer, the second most common worldwide, causes significant morbidity and mortality in developing nations. Aberrant HSP-27 expression links to aggressive tumors, chemotherapy resistance, and poor prognosis. The stage of disease at diagnosis is the most important prognostic variable. Hence, this study was undertaken to evaluate the expression of HSP-27 in Invasive breast carcinoma and metastatic regional lymph nodes.

Methods: The present study was a prospective study conducted from December 2019 to May 2021. Data was obtained from clinical records of 35 patient’s preliminary diagnosed as breast carcinoma by different diagnostic modalities Corresponding modified radical mastectomy specimens were grossed according to standard protocol ,processed to paraffin block and sectioned. The slides were stained with routine Haematoxylin and eosin stain. Additionally, the slides were immunostained with HSP-27 antibody.

Results: The study included 35 female patients aged 29 to 80 years, with a mean age of 50 years. Most cases (37%) were between 31–40 years. Invasive ductal carcinoma, no special type, was the most common histopathologic pattern (97%). Grade II tumors (46%) and TNM stage IIIA (34%) predominated. Positive HSP-27 expression was noted in 89% of breast carcinomas and 100% of metastatic lymph nodes. Expression intensity varied, but no significant correlation was found with tumor grade or stage (p > 0.05).

Conclusion: HSP-27 can be a reliable tumor marker in breast carcinoma and may be a molecular target in cancer therapy.

Keywords: breast carcinoma; metastatic lymph node; heat shock protein-27